吴中柳

职 务：重点实验室主任助理
职 称：研究员
学位：博士
电 话：028-82890423, 028-82890434
电子邮件:wuzhl@cib.ac.cn

个人简历

　　吴中柳，研究员，女，1975年6月生。2003年毕业于中国科学院上海有机化学研究所生物有机化学国家重点实验室，获理学博士学位，同年赴美国Vanderbilt大学医学院生物化学系从事博士后研究工作，2006年入选中国科学院“百人计划”(“引进国外杰出人才”) ，2008年获得择优支持。现任中国科学院成都生物研究所应用与环境微生物研究中心研究员，博士生导师。入选2008年度四川省杰出青年学科带头人。

研究方向

　　主要从事生物催化与生物转化研究，包括以下几方面：

　　1、新型微生物氧化还原酶酶源的筛选、评估和应用；

　　2、酶催化剂的分子改造和进化；

　　3、重要手性分子的酶催化合成新方法；

　　4、生物催化法构建多样性的活性分子库和新型类药分子。

课题组网页：http://www.emlab.cib.ac.cn/document.action?docid=44616

 

代表论著

1. Zhao, F.-J.; Pei, X.-Q.; Ren, Z.-Q.; Wu, Z.-L.* Rapid asymmetric reduction of ethyl 4-chloro-3-oxobutanoate using a thermostabilized mutant of ketoreductase ChKRED20. Appl Microbiol Biotechnol. 2015. Doi：10.1007/s00253-015-7200-2

2. Li, J.-J.; Pei, X.-Q.; Zhang, S.-B.; Wu, Z.-L*. Improving the thermostability of feruloyl esterase by DNA shuffling and site-directed mutagenesis. Process Biochemistry. 2015, 50: 1783-1787.

3. Li, J.-J.; Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L*. Removal of the free cysteine residue reduces irreversible thermal inactivation of Feruloyl Esterase: evidence from circular dichroism and fluorescence spectra. Acta Biochimica et Biophysica Sinica. 2015, 47(8):612-619. Doi：10.1093/abbs/gmv057

4. Liu, Y.-C; Wu, Z.-L*. Switchable asymmetric bio-epoxidation of a,b-unsaturated ketones. Chem. Commun. 2015, DOI: 10.1039/C5CC07548C

5.  Ren, Z.-Q; Liu, Y.; Pei, X.-Q; Wang H.-B.; Wu, Z.-L*. Bioreductive production of enantiopure (S)-duloxetine intermediatescatalyzed with ketoreductase ChKRED15. J. Mol. Catal. B: Enzym. 2015, 113,76–81.

6. X.-Q. Pei; M.-Y. Xu; Wu, Z.-L.* Two “classical” Old Yellow Enzymes from Chryseobacterium sp. CA49: broad substrate specificity of Chr-OYE1 and limited activity of Chr-OYE2.  J. Mol. Catal. B: Enzym. 2016, 123:91-99.

7.  Liu, Y.-C; Liu, Y.; Wu, Z.-L*. Synthesis of enantiopure glycidol derivatives via a one-pot two-step enzymatic cascade.  Org. Biomol. Chem., 2015, 13, 2146–2152

8. Xu, M.-Y., Pei, X.-Q., Wu, Z.-L.* Identification and characterization of a novel "thermophilic-like" Old Yellow Enzyme from the genome of Chryseobacterium sp. CA49. J. Mol. Catal. B: Enzym. 2014, 108, 64-71.

9. Tang, T.-X.; Liu, Y; Wu, Z.-L.* Characterization of a robust anti-Prelog short-chain dehydrogenase/reductase ChKRED20 from Chryseobacterium sp. CA49. J. Mol. Catal. B: Enzym. 2014, 105, 82-88.

10. Liu, Y., Tang, T.-X., Pei, X.-Q., Zhang, C., Wu, Z.-L.* Identification of ketone reductase ChKRED20 from the genome of Chryseobacterium sp. CA49 for highly efficient anti-Prelog reduction of 3,5-bis(trifluoromethyl)acetophenone. J. Mol. Catal. B: Enz. 2014, 102(4), 1-8.

11. Wang, H.-B., Pei, X.-Q.; Wu, Z.-L.* An enoate reductase Acrh-OYE4 from Achromobacter sp. JA81: Characterization and application in asymmetric bioreduction of C=C bonds. Appl. Microbiol. Biotechnol. 2014, 98(2), 705-715.

12. Yi, Z.-L.; Zhang, S.-B.*; Pei, X.-Q.; Wu, Z.-L.* Design of mutants for enhanced thermostability of b-glycosidase BglY from Thermus thermophilus. Bioresour. Technol. 2013, 129, 629-633.

13. Lin, H.; Tang, D.-F.; Ahmed, A. A. Q.; Liu, Y.; Wu, Z.-L.* Mutations at the putative active cavity of styrene monooxygenase: enhanced activity and reversed enantioselectivity. J. Biotechnol. 2012, 161, 235-241.

14. Zhang, S.-B.; Pei, X.-Q.; Wu, Z.-L.* Multiple amino acid substitutions significantly improve the thermostability of feruloyl esterase A from Aspergillus niger. Bioresour. Technol. 2012, 117, 140-147.

15. Liu, Y.-J.; Pei, X.-Q.; Lin, H.; Gai, P.; Liu, Y.-C.; Wu, Z.-L.* Asymmetric bioreduction of activated alkenes by a novel isolate of Achromobacter species producing enoate reductase. Appl. Microbiol. Biotechnol. 2012, 95(3), 635-645.

16. Zhang, S.-B.; Ma, X.-F.; Pei, X.-Q.; Liu, J.-Y.; Shao, H.-W.*, Wu, Z.-L.* A practical high-throughput screening system for feruloyl esterases: substrate design and evaluation. J. Mol. Catal. B: Enzym. 2012, 74, 36-40.

17. Lin, H.; Liu, J.-Y.; Wang, H.-B.; Ahmed, A. A. Q.; Wu, Z.-L.* Biocatalysis as an alternative for the production of chiral epoxides: a comparative review. J. Mol. Catal. B: Enzym. 2011, 72(3-4), 77-89. (Invited review)

18. Tang, C.-G.; Lin, H.; Zhang, C.; Liu, Z.-Q.; Yang, T.; Wu, Z.-L.* Highly enantioselective bioreduction of N-methyl-3-oxo-3- (thiophen-2-yl) propanamide for the production of (S)-duloxetine.  Biotechnol. Lett. 2011, 33(7), 1435-1440.

19. Lin, H.; Liu, Y.; Wu, Z.-L.* Highly diastereo- and enantio-selective epoxidation of secondary allylic alcohols catalyzed by styrene monooxygenase. Chem. Commun., 2011, 47, 2610-2612.

20. Lin, H.; Liu, Y.; Wu, Z.-L.* Asymmetric epoxidation of styrene derivatives by styrene monooxygenase from Pseudomonas sp. LQ26: effects of a- and b-substituents. Tetrahedron: Asymmetry, 2011, 22, 134-137.

21. Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L.* Introduction of glycine and proline residues onto protein surface increases the thermostability of endoglucanase CelA from Clostridium thermocellum. Bioresour. Technol. 2011, 102(3), 3636–3638

22. Pei, X.-Q.; Yi, Z.-L.; Tang, C.-G.; Wu, Z.-L.* Three amino acid changes contribute markedly to the thermostability of b-glucosidase BglC from Thermobifida fusca. Bioresour. Technol. 2011, 102(3), 3337–3342.

23. Qaed, A. A.; Lin, H.; Tang, D.-F.; Wu, Z.-L.* Rational design of styrene monooxygenase mutants with altered substrate preference. Biotechnol. Lett. 2011, 33, 611-616.

24. Zhang, S.-B.; Wu, Z.-L.* Identification of amino acid residues responsible for increased thermostability of feruloyl esterase A from Aspergillus niger using the PoPMuSiC algorithm.  Bioresour. Technol. 2011, 102 (2), 2093–2096.

25. Lin, H.; Qiao, J.; Liu, Y.; Wu, Z.-L.* Styrene monooxygenase from Pseudomonas sp. LQ26 catalyzes the asymmetric epoxidation of both conjugated and unconjugated alkenes. J. Mol. Catal. B: Enzym. 2010, 67(3-4), 236-241.

26. Zhang, Z.-G.; Yi, Z.-L.; Pei, X.-Q.; Wu, Z.-L.* Improving the thermostability of Geobacillus stearothermophilus xylanase XT6 by directed evolution and site-directed mutagenesis. Bioresour. Technol. 2010, 101(23), 9272-9278.

27. Yi, Z.-L.; Wu, Z.-L.* Mutations from a family-shuffling-library reveal amino acid residues responsible for the thermostability of endoglucanase CelA from Clostridium thermocellum Biotechnol. Lett. 2010, 32(12), 1869-1875.

28. Tang, Z., Salamanca-Pinzon, S.G., Wu, Z.L., Xiao, Y., Guengerich, F.P., Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function. Arch. Biochem. Biophys. 2010, 494(1), 86-93.

29. Wu, Z.-L.*; Qiao, J.; Zhang, Z.-G.; Guengerich, F.P.; Liu, Yan; Pei, X.-Q. Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression. Biotechnol. Lett. 2009, 31(10), 1589-1593.

30. Zhang, Z.-G.; Liu, Y.; Guengerich, F.P.; Matse, J.H.; Chen J.; Wu, Z.-L.* Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries. J. Biotechnol. 2009, 139(1), 12-18.

31. Stark, K.; Wu, Z.-L.; Bartleson, C.J.; Guengerich, F.P. Heterologous expression and mRNA distribution of orphan cytochrome P450 20A1. Drug Metab. Dispos. 2008, 36(9), 1930-1937.

32. Wu, Z.-L.; Sohl, C.D.; Shimada, T.; Guengerich, F.P. Recombinant Enzymes Over expressed in Bacteria Show Broad Catalytic Specificity of Human Cytochrome P450 2W1 and Limited Activity of Human Cytochrome P450 2S1. Mol. Pharmacol., 2006, 69(6), 2007-2014.

33. Wu, Z.-L.; Bartleson, C.J.; Ham, A.J.; Guengerich, F.P. Heterologous Expression, Purification, and Properties of human cytochrome P450 27C1, Arch. Biochem. Biophys. 2006, 445(1), 138-146.

34. Wu, Z.-L.; Podust, L.M.; Guengerich, F.P. Expansion of Substrate Specificity of Cytochrome P450 2A6 by Random and Site-Directed Mutagenesis. J. Biol. Chem. 2005, 280(49), 41090-41100.

35. Kim, D.; Wu, Z.-L.; Guengerich, F.P. Analysis of coumarin 7-hydroxylation activity of cytochrome P450 2A6 using random mutagenesis. J. Biol. Chem. 2005, 280(48), 40319-40327.

36. Wu, Z.-L.; Aryal, P.; Lozach, O.; Meijer, L.; Guengerich, F.P. Biosynthesis of New Indigoid Inhibitors of Protein Kinases Using Recombinant Cytochrome P450 2A6, Chem. Biodiv. 2005, 2(1), 51-65.

37. Guengerich, F.P.; Wu, Z.-L.; Bartleson, C.J. Function of Human Cytochrome P450s: Characterization of the Orphans. Biochem. Biophys. Res. Commun. 2005, 338(1), 465-469.

38. Wu, Z.-L.; Li, Z.-Y. Highly Enantioselective Synthesis of a,a-Disubstituted Malonamic Acids through Asymmetric Hydrolysis of Dinitriles with Rhodococcus sp. CGMCC 0497, Chem. Commun. 2003, 3, 386-387. (Selected by ChemInform, 2003, 34(23), Nitriles Q0520.)

39. Wu, Z.-L.; Li, Z.-Y. Practical Synthesis of Optically Active a,a-Disubstituted Malonamic Acids through Asymmetric Hydrolysis of Malonamide Derivatives with Rhodococcus sp. CGMCC 0497, J. Org. Chem. 2003, 68(6), 2479-2482.

40. Wu, Z.-L.; Li, Z.-Y. Biocatalytic Asymmetric Hydrolysis of (±)-b-Hydroxy Nitriles by Rhodococcus sp. CGMCC 0497, J. Mol. Catal. B: Enzym. 2003, 22(1-2), 105-112.

41. Wu, Z.-L.; Li, Z.-Y. Enantioselective Biotransformation of a,a-Disubstituted Dinitriles to the Corresponding 2-Cyano-Acetamides using Rhodococcus sp. CGMCC 0497, Tetrahedron: Asymmetry, 2003, 14(15), 2133-2142.

42. Wu, Z.-L.; Li, Z.-Y. Enhancement of Enzyme Activity and Enantioselectivity via Cultivation in Nitrile Metabolism by Rhodococcus sp. CGMCC 0497, Biotechnol. Appl. Biochem. 2002, 35(1), 61-67.

43. Wu, Z.-L.; Li, Z.-Y. Enantioselective Hydrolysis of Various Racemic a-Substituted Arylacetonitriles using Rhodococcus sp. CGMCC 0497, Tetrahedron: Asymmetry 2001, 12(23), 3305-3312.

44. Li, Z.-Y., Wu, Z.-L., Chen, Y. (李祖义，吴中柳，陈颖) Evolution of Industrialization of Biocatalysis (生物催化产业化进展), Chin. J. Org. Chem. (有机化学) 2003, 23(12), 1446-1451.

45. Li, Z.-Y., Wu, Z.-L., Li, J. (李祖义，吴中柳，励俊) New Methods in Immobilization: Biocapsulation by Epoxy-amine Resins and Silicone Elastomers (新型固定化方法－环氧胺树脂及硅树脂生物包埋) Chin. J. Org. Chem. (有机化学) 2003, 23, 150-154.

46. Wu, Z.-L.; Li, Z.-Y. (吴中柳，李祖义) Microbial and Enzymatic Hydrolysis of Nitriles in Organic Synthesis (氰基水解酶在有机合成中的应用), Chin. J. Org. Chem. (有机化学) 2001, 21, 25-32.

 

授权专利　　

　　1. 吴中柳，刘艳，裴小琼；一种新型羰基还原酶、其基因及应用. 专利号:ZL 201410012867.9;申请日: 2014.01.12;授权日:2015.12.9.

　　2. 吴中柳，刘艳，汤脱险，裴小琼；一株金黄杆菌及其羰基还原酶用于阿瑞匹坦手性中间体生产. 专利号ZL201310399109.2；申请日：2013.09.05；授权日：2015.03.25.

　　3. 吴中柳，张志刚，刘艳；一种1, 4-b-D-木聚糖酶突变体（分案申请）.专利号: ZL201210426297.9；申请日：2010.05.31；授权日: 2014.12.24.

　　4. 吴中柳，刘艳，张超，汤传根，田小亮，裴小琼；氧化微杆菌及其制备手性双三氟甲基苯乙醇的方法. 专利号: ZL201010271579.7；申请日: 2010.09.03; 授权日: 2013.02.13.

　　5. 吴中柳，张帅兵，裴小琼，刘艳；一种阿魏酸酯酶A突变体及其用途. 专利号: ZL201110102026.3；申请日: 2011.04.22; 授权日: 2013.03.13.

　　6. 吴中柳，张志刚，刘艳；一种1, 4-b-D-木聚糖酶突变体. 专利号: ZL201010187324.2；申请日：2010.05.31；授权日: 2013.06.05.

　　7. 吴中柳，刘艳杰，林晖；一株无色杆菌及其不对称催化还原碳碳双键的方法. 专利号: ZL201210004039.1；申请日: 2012.01.08；授权日: 2013.06.05.

　　8. 吴中柳，林晖，刘艳，乔婧；一种苯乙烯环氧化酶基因及其用途，专利号:ZL201010120969.4；申请日：2010.03.10，授权日：2014.09.17.

　　9. 吴中柳，汤传根，张超，杨涛，刘章琴，林晖；一株粘红酵母及其制备(S)-羟基丙酰胺的方法，专利号ZL201010509822.4；申请日：2010.10.15；授权日2014.03.12.

　　10. 李祖义，吴中柳；Rhodococcus sp. D12 and Its Culture Method and Application, CN 1128874C, ZL 00127452.X, CN 1291648 A 18 Apr 2001, 17 pp. 2003.11.26

　　

承担科研项目情况

国家自然科学基金（2008-2010，2011-2013 & 2014-2017）

科技部科技重大专项

科技部中国越南政府间国际合作

中国科学院百人计划

中国科学院知识创新工程重大项目专题

四川省青年科技基金

四川省科技基金

企业横向委托项目